A recent study used digital spatial profiling and multiplex IHC to study the immune responses of COVID-19/HIV co-infected or COVID-19-infected patients. The authors found a diminished T-cell response but an increased myeloid cell response associated with SARS-CoV-2 viral load (NP+) in the liver and the kidney of the COVID-19/HIV patient. These findings suggest that HIV as a comorbidity promotes virus-induced myeloid cell activation in response to SARS-CoV-2 infection in the absence of a viable T-cell response.
The complex interactions between tumor, immune, and other cells in the tumor microenvironment (TME) affect the efficacy of cancer immunotherapy. The Nolan Lab from Stanford University has developed a multiplexed tissue imaging platform called CO-Detection by indEXing (CODEX) that can detect up to 60 markers simultaneously in the same tissue sections at the single-cell level. They recently used the technology to develop a 56-marker panel and use it to characterize the tumor samples from eight cutaneous T-cell lymphoma (CTCL) patients. The marker panel provides a blueprint to study tumor immunology, tissue architecture, and mechanisms of immunotherapeutic targets.
Standard reference images are important to standardize and benchmark different staining and imaging protocols. JEDI is a multi-disciplinary taskforce with more than 30 members across the world, working closely with the Society of Immunotherapy for Cancer (SITC) and World Immunotherapy Council (WIC) to help the standardization and translation of multiplex IHC/IF technologies for clinical research and cancer immunotherapy application.
A study based on simultaneous detection and quantification of three different programmed death-ligand 1 (PD-L1) antibodies found moderate-to-strong correlations between their staining levels in conventional immunohistochemistry (IHC) and multiplex immunohistochemistry/ immunofluorescence (mIHC/IF).
- ImmunoAtlas Report for the study (ATLA-201129-1)
A study showed that quantifications of CD38+ cells and CD38+CD68+ macrophages can more accurately predict immune-checkpoint-blockade responsiveness than quantifications based on programmed death-ligand 1 (PD-L1) or CD8+ T cells only.
- ImmunoAtlas Report for the study (ATLA-201126-1)
A new study performed whole-slide multiplexed immunohistochemistry and immunofluorescence (mIHC/IF) and hematoxylin and eosin (H&E) staining and imaging on the same human tonsil section. This is a promising method to simultaneously and spatially quantify overall tissue morphology and various molecular phenotypes of cancer and immune cells in the tumor microenvironments.