|Report name||CCTL Front. Immunol.|
|Study name||Highly Multiplexed Phenotyping of Immunoregulatory Proteins in the Tumor Microenvironment by CODEX Tissue Imaging|
|Study authors||Nolan Lab|
Immunotherapies are revolutionizing cancer treatment by boosting the natural ability of the immune system. To better inform clinical care and the design of therapeutic combination strategies, the co-expression of immunoregulatory proteins on individual immune cells within the tumor microenvironment must be robustly characterized. Highly multiplexed tissue imaging platforms, such as CO-Detection by indEXing (CODEX), are primed to meet this need by enabling >50 markers to be simultaneously analyzed in single-cells on formalin-fixed paraffin-embedded (FFPE) tissue sections. Herein, we report the design, development, optimization, and application of a 56-marker CODEX antibody panel to eight cutaneous T cell lymphoma (CTCL) patient samples. This panel is comprised of structural, tumor, and immune cell markers, including eight immunoregulatory proteins that are approved or currently undergoing clinical trials as immunotherapy targets. This study provides a resource to enable extensive high-dimensional, spatially resolved characterization of the tissue microenvironment across tumor types and imaging modalities, while enabling researches to gain mechanistic insights into immunotherapeutic targets.
|Specimen type||Human skin tissue|
(Nolan Lab, Pathology - Baxter Laboratory, Stanford)
|Last updated||10 Jan, 2022 (Mon),